| 1 | Hammer Bro | Super Mario Bros., Super Mario Galaxy, Super Mario Odyssey | Unpredictable hammers, fast movement | Jump over and stomp or use fireballs |
| 2 | Magikoopa | Super Mario World, Super Mario Galaxy, New Super Mario Bros. U | Teleports, casts damaging magic | Jump on or use power-ups quickly |
| 3 | Chargin' Chuck | Super Mario World, Super Mario 3D World | High HP, aggressive, multiple attacks | Jump multiple times or use capes/fire |
| 4 | Boos | Super Mario Bros. 3, Super Mario 64, Luigi's Mansion series | Invincible when watched, can swarm | Turn away, use star or environmental kills |
| 5 | Thwomp | Super Mario Bros. 3, Super Mario 64, Super Mario Galaxy | Crushes instantly, appears suddenly | Wait for drop, then run or jump past |
| 6 | Fuzzy | Paper Mario, New Super Mario Bros. U | Drains HP and multiplies | Attack early and use badges |
| 7 | Spinies | Super Mario Bros., Super Mario 64, Super Mario Galaxy | Can't be jumped on, often spammed | Use fireballs, shells, or projectiles |
| 8 | Pokey | Super Mario Bros. 2, Paper Mario, New Super Mario Bros. Wii | Tall, segmented, hard to jump over | Hit with fire or Yoshi eats them |
| 9 | Chain Chomp | Super Mario Bros. 3, Super Mario 64, Paper Mario | Fast, high damage, breaks free | Dodge and use terrain; jump in RPGs |
| 10 | Mechakoopa | Super Mario World, Super Mario Galaxy 2 | Explodes when thrown, mechanical | Jump to stun, throw into hazards |
| 11 | Monty Mole | Super Mario World, Super Mario 64, New Super Mario Bros. Wii | Pops up suddenly, fast | Stay mobile, jump when they pop |
| 12 | Fire Bro | Super Mario Bros. 3, New Super Mario Bros. U | Throws fireballs rapidly | Jump behind or use fire/cape |
| 13 | Sledge Bro | Super Mario Bros. 3, New Super Mario Bros. Wii | Ground pounds shake screen | Time jumps, fireballs from afar |
| 14 | Bully | Super Mario 64, Super Mario 64 DS | Pushes Mario off platforms | Push them back or use knockback moves |
| 15 | Banzai Bill | Super Mario World, New Super Mario Bros. U | Giant size, fast, hard to avoid | Jump over or duck quickly |
| 16 | Dry Bones | Super Mario Bros. 3, New Super Mario Bros. Wii, Super Mario Maker | Revives unless burned | Use fireballs, POW blocks |
| 17 | Big Goomba | Super Mario 64 DS, New Super Mario Bros. | Huge hitbox, requires multiple hits | Stomp from above repeatedly |
| 18 | Anger Wiggler | Paper Mario: The Thousand-Year Door | Much more aggressive and faster than normal Wiggler | Use timed attacks and defensive buffs to survive its fury |
| 19 | Wiggler | Super Mario World, Super Mario 64, Paper Mario | Turns aggressive when provoked | Jump or attack while calm, time hits |
| 20 | Shroob UFO | Mario & Luigi: Partners in Time | Strong lasers, resists normal attacks | Use specific bro attacks and badges |